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recombinant human netrin 1 his  (R&D Systems)


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    Structured Review

    R&D Systems recombinant human netrin 1 his
    Recombinant Human Netrin 1 His, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+netrin+1/Recombinant+Human+Netrin-1+Protein/pmc12711026-341-8-12
    Average 93 stars, based on 56 article reviews
    recombinant human netrin 1 his - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Recombinant:

    Article Title: Netrin signaling mediates survival of dormant epithelial ovarian cancer cells
    Article Snippet: Sequence-based reagent , DSCAM-1sgRNA , GeCKO genomic CRISPRLibrary V2 , CRISPR guide , 5’-ATCGTAGATC TCCTCGCCCG-3’. .. Peptide, recombinant protein , Recombinant Human Netrin-1 , R and D Systems , #6419-N1 , 500 ng/ml. .. Commercial assay or kit , Monarch DNA Gel Extraction Kit , New England Biolabs , #T1020 , .

    Article Title: Netrin-1 feedforward mechanism promotes pancreatic cancer liver metastasis via hepatic stellate cell activation, retinoid, and ELF3 signaling.
    Article Snippet: .. The next day, 100,000 cells in serum starvation mediumwere placed in the top of a transwell (8 mmfilter, Falcon, 353097), with the bottom chamber containing either SSM, SSM+ 150 ng/mL recombinant human Netrin-1 in PBS/0.2% BSA (R&D Systems, 1109-N1-025), or medium +10% FBS. .. For invasion assays, transwells were coated with 1 mg/mL growth factor reduced matrigel (Corning) and allowed to solidify 1 h at 37 C prior to loading cells into the chamber.

    Article Title: Photolithography-Based Substrate Microfabrication for Patterning Semaphorin 3A to Study Neuronal Development
    Article Snippet: .. The same composition of stripe patterning solution can be used to pattern other extracellular proteins, with suggested final concentrations: 0.5 ng/ml Recombinant human BDNF and NGF (PeproTech Inc., Rocky hill, NJ), 0.5 or 0.05 ng/ml Recombinant human netrin-1 (R&D Systems) ( see Note 4 ). ..

    Article Title: Netrin-1 feedforward mechanism promotes pancreatic cancer liver metastasis via hepatic stellate cell activation, retinoid, and ELF3 signaling
    Article Snippet: .. The next day, 100,000 cells in serum starvation medium were placed in the top of a transwell (8 μm filter, Falcon, 353097), with the bottom chamber containing either SSM, SSM+ 150 ng/mL recombinant human Netrin-1 in PBS/0.2% BSA (R&D Systems, 1109-N1-025), or medium +10% FBS. .. For invasion assays, transwells were coated with 1 mg/mL growth factor reduced matrigel (Corning) and allowed to solidify 1 h at 37°C prior to loading cells into the chamber.

    Article Title: UNC5B Overexpression Alleviates Peripheral Neuropathic Pain by Stimulating Netrin-1-Dependent Autophagic Flux in Schwann Cells.
    Article Snippet: Lesions or diseases of the somatosensory system can cause neuropathic pain (NP).. Schwann cell (SC) autophagy plays an important role in NP.. Uncoordinated gene 5 homolog B (UNC5B), the canonical dependent receptor of netrin-1, is known to be exclusively expressed in SCs and involved in NP; however, the underlying mechanisms were unclear.

    Article Title: Netrin‐1 and its receptor DCC modulate survival and death of dopamine neurons and Parkinson’s disease features
    Article Snippet: .. Recombinant human netrin‐1 (R&D) and GDNF (PeproTech) were iodinated with 125 I‐Na using the lactoperoxidase method. .. Proteins were dissolved in 30 μl of 0.25 M phosphate buffer, pH 7.5, and mixed with 125I ‐Na (1 mCi 37 mBq; GE Healthcare).

    Article Title: Netrin-1 acts as a non-canonical angiogenic factor produced by human Wharton's jelly mesenchymal stem cells (WJ-MSC).
    Article Snippet: .. The experimental approaches included: DMEM (20 μL), 2F5 (0.5 μg/μL, kindly provided by Dr. Mehlen), CBO (50 μM, Calbiochem), C3 transferase (Cytoskeleton, Inc., CT04 1–1.5 μg/mL) and recombinant human Netrin-1 (R&D Systems, 10 ng/mL). ..

    Article Title: FANCC localizes with UNC5A at neurite outgrowth and promotes neuritogenesis
    Article Snippet: SH-SY5Y cells (ATCC, CRL-2266) were grown in a mixture of DMEM and Ham’s F12 Nutrient Mixture (1:1) with 10% FCS at 37 °C, 5% CO2, followed by transfection using calcium-phosphate or lipofectamine 2000 (ThermoFisher Scientific). .. For differentiation assays, SH-SY5Y cells were treated with retinoic acid (10 μM; Sigma-Aldrich) for 48 h or recombinant human Netrin-1 (500 ng/ml, R&D systems, #6419-N1) for 4 h prior to immunofluorescence staining. ..

    Concentration Assay:

    Article Title: UNC5B Overexpression Alleviates Peripheral Neuropathic Pain by Stimulating Netrin-1-Dependent Autophagic Flux in Schwann Cells.
    Article Snippet: Lesions or diseases of the somatosensory system can cause neuropathic pain (NP).. Schwann cell (SC) autophagy plays an important role in NP.. Uncoordinated gene 5 homolog B (UNC5B), the canonical dependent receptor of netrin-1, is known to be exclusively expressed in SCs and involved in NP; however, the underlying mechanisms were unclear.

    Immunofluorescence:

    Article Title: FANCC localizes with UNC5A at neurite outgrowth and promotes neuritogenesis
    Article Snippet: SH-SY5Y cells (ATCC, CRL-2266) were grown in a mixture of DMEM and Ham’s F12 Nutrient Mixture (1:1) with 10% FCS at 37 °C, 5% CO2, followed by transfection using calcium-phosphate or lipofectamine 2000 (ThermoFisher Scientific). .. For differentiation assays, SH-SY5Y cells were treated with retinoic acid (10 μM; Sigma-Aldrich) for 48 h or recombinant human Netrin-1 (500 ng/ml, R&D systems, #6419-N1) for 4 h prior to immunofluorescence staining. ..

    Staining:

    Article Title: FANCC localizes with UNC5A at neurite outgrowth and promotes neuritogenesis
    Article Snippet: SH-SY5Y cells (ATCC, CRL-2266) were grown in a mixture of DMEM and Ham’s F12 Nutrient Mixture (1:1) with 10% FCS at 37 °C, 5% CO2, followed by transfection using calcium-phosphate or lipofectamine 2000 (ThermoFisher Scientific). .. For differentiation assays, SH-SY5Y cells were treated with retinoic acid (10 μM; Sigma-Aldrich) for 48 h or recombinant human Netrin-1 (500 ng/ml, R&D systems, #6419-N1) for 4 h prior to immunofluorescence staining. ..



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